GeneDetect.com
Our Products Setup or update your Account Frequently Asked Questions About Our Company How you may contact us Search our Product database or the website Return to GeneDetect.com's homepage Shopping Basket Contents Get a valid online quote Checkout
GeneDetect Laboratory Protocols  


Laboratory Protocols for Download.

NEW. Updated 15 October, 2014. This page will be updated frequently with protocols optimized for use with the GeneDetect® range of products for radioactive and non-radioactive in situ hybridization. 

Please download protocols below.

NEW. Use Google scholar to search for in situ hybridization protocol papers to help you get your in situ hybridization experiments working well. For example, search for earlier published articles using GeneDetect probes by searching for GeneDetect or search for in situ hybridization optimization to find optimized in situ hybridization protocols. Of course, please also contact us directly for direct technical support. Google scholar is a great resource. Use it!!

NEW. Note you can currently find > 350 recent publications using GeneDetect products by typing GeneDetect into the link below.

Google Scholar


GeneDetect® oligonucleotide probes.

1. Handling and storage instructions.

 

In situ hybridization protocols.

FROZEN SECTIONS
1. 35S-labeled GeneDetect® probe, Detection by emulsion or film autoradiography. 
2. GreenStar* FITC-hyperlabeled probe, Detection by direct fluorescence, AP (alkaline phosphatase) or tyramide signal amplification (TSA). 
3. GreenStar* DIG-hyperlabeled probe, Detection by direct fluorescence, AP (alkaline phosphatase) or tyramide signal amplification (TSA). 
4. GreenStar* BIOTIN-hyperlabeled probe, Detection by AP (alkaline phosphatase) or HRP (Horseradish Peroxidase) with or without tyramide signal amplification (TSA).

PARAFFIN SECTIONS
1. GreenStar* BIOTIN-hyperlabeled probe, Detection by AP (alkaline phosphatase) or HRP (Horseradish Peroxidase) with or without tyramide signal amplification (TSA).
2. GreenStar* DIG-hyperlabeled probe, Detection by direct fluorescence, AP (alkaline phosphatase) or tyramide signal amplification (TSA).
3. DIG-labeled GeneDetect® probe, Detection by AP (alkaline phosphatase), FITC or rhodamine.


Use the FREE Acrobat Reader available from the Adobe website to read PDF files. Are you having problems reading the .pdf files? Click here


GeneDetect® is a registered trademark of GeneDetect.com Limited 
(USPTO Reg 2765935).  

The use of oligonucleotide probes for localizing mRNA in tissue sections is licensed from Syngene (Australia). The Syngene "In situ Hybridization" patents licensed include US Patents 5,597,692 and 6,265,156, European Patent 0,175,776, Canadian Patent 1,251,119, Australian Patent 579,631 and Japanese Patent 1,970,487. § The use of digoxigenin technology to label nucleic acids is licensed under patents (EP 0 324 474, US 5.344.757, US 5.702.888, US 5.354.657, JP 1999884 and HK 1169) owned by Roche Diagnostics GmbH. For further information on products to label and detect digoxigenin-modified nucleic acids refer to www.roche-applied-science.com/dig/

 

Copyright © 2000-2014 GeneDetect.com Limited